Hach-lange BODTRAK II: Respirometric Biochemical Oxygen Deman Manual de usuario

DOC022.53.90072BODTrak™ IIUSER MANUAL 02/2010,Edition 2© Hach Company, 2008, 2010. All rights reserved. Printed in China.

10

11Section 3 Installation3.1 Component listCompare each item below to the items in the shipment. If an item is missing or damaged, refer to the manufac

Installation123.2 Electrical installationThe power adaptor supplies AC power to the IEC universal connector (Figure 1). The power switch powers the in

13Section 4 Operation4.1 Operational controlsThe BODTrak™ II operator controls are shown in Figure 2. Figure 2 Operational controls1 Display 4 ON/OFF

Operation144.1.1 Channel selection keysPush the related channel selection key to show data for one of the 6 bottles. The channel selection keys are al

Operation154.3 Setting the clockAll channels must show END or CLEAR before the clock can be set. Push and hold the two arrow keys at the same time unt

Operation164.5 Downloading test resultsTo transfer test results to a PC:1. Choose PROGRAMS, ACCESSORIES, COMMUNICATIONS, HYPERTERMINAL.2. In the Conne

Operation174.5.2 Data formatWhen a result array is downloaded to HyperTerminal, all data from the test are sent without pause. The data flow cannot be

Operation184.5.3 Printing test resultsThe BODTrak II is compatible with the Citizen PD-24 printer, which is available as an optional accessory (Sectio

19Section 5 BODTrak™ II procedures5.1 General InformationThere are three BODTrak II procedure variations. Choose the procedure that meets the applicat

BODTrak™ II procedures205.2 Simplified procedureRequired apparatus:BODTrak II bottleThermometerBlender (optional)Graduated cylinderRequired reagents:1

BODTrak™ II procedures214. Add the contents of 1 nutrient buffer pillow to the graduated cylinder. 5. Transfer the contents of the graduated cylinder

BODTrak™ II procedures225.3 Hach GGA (glucose/glutamic acid) procedureRequired apparatus:BODTrak II bottleGraduated cylinderVolumetric pipet and pipet

BODTrak™ II procedures23Table 5 GGA sample volumesBOD range (mg/L)GGA volume (mL)Seed volume (mL)Final Volume (mL)0 to 350 8.0 10 to 35 160Note: If s

BODTrak™ II procedures245.4 Hach Standard Method procedureRequired apparatus:ThermometerBODTrak II bottleBlender (optional)Graduated cylinderTensette

BODTrak™ II procedures251. Heat or cool the sample to 19 to 21 ºC(66 to 70 ºF).2. Homogenize the sample in a blender if it contains large settleable

BODTrak™ II procedures267. Fill to the final test range volume, if necessary, with a deionized wash water bottle (Table 6 on page 24). 8. Transfer the

BODTrak™ II procedures275.5 Completion steps for all proceduresRequired apparatus:BODTrak IISpatula scoop BOD incubator Seal cupStir barRequired reage

BODTrak™ II procedures284. Put the bottles on the BODTrak II chassis. Connect the applicable tube to the sample bottle and tighten the cap.5. Put the

BODTrak™ II procedures2910.To start the test, push the channel number applicable to the bottle. 11.Push the ON key. The range selection menu is shown.

3Table of contentsSection 1 Specifications ... 5Section 2 General info

BODTrak™ II procedures305.5.1 Determination of resultsAfter the end of the chosen test period (5, 7 or 10 days), END is shown on the display. The proc

BODTrak™ II procedures314. When samples are seeded, calculate the results using this equation and the corrected results:Where: A = corrected BOD of th

BODTrak™ II procedures325.6 Typical curvesTypical curves through a 10 day test period are shown in Figure 4. For incorrect curves refer to Figure 5 on

BODTrak™ II procedures335.7 Special considerations5.7.1 Sample dilutionUnknown sample BOD effluent is typically in the 0 to 70 mg/L range. Unknown sam

BODTrak™ II procedures345.7.5 ChlorineAny chlorine in the sample must be removed prior to testing. Keep the sample at room temperature for 1 to 2 hour

35Section 6 MaintenanceDANGEROnly qualified personnel should conduct the tasks described in this section of the manual. 6.1 Cleaning the instrumentCle

36

37Section 7 TroubleshootingIncorrect BOD curves through a 10 day test period are shown in Figure 5. For typical curves refer to Figure 4 on page 32.Fi

Troubleshooting387.1 High oxygen demandSamples that are above range (for example, a BOD over 350 mg/L when a 160-mL sample is taken) will cause result

Troubleshooting397.5 Bottle leakCurve 5 (Figure 5 on page 37) shows a bottle leak. A bottle leak can also cause no response from the system. If such a

Table of contents46.1.2 Stir bars and seal cups ...356.1.3 Bottle fences ...

40

41Section 8 Replacement parts and accessories8.1 Replacement partsDescription Quantity Item NumberBODTrak™ II Instrument, 115/230 VAC 1 2952400Bottle,

42Sulfuric acid, 0.02 N standard solution, 1000 mL 1 20353Sulfuric acid, 1.0 N standard solution, 1000 mL1 127053Voluette ampule standard for BOD, 300

Replacement parts and accessories43Thermometer, Mercury, -20 to 110 °C 1 56601Thermometer, non-Mercury, -20 to 110 °C 1 2635702Water Still, 120 V 1 2

44

45Section 9 Contact InformationHACH Company World HeadquartersP.O. Box 389Loveland, Colorado80539-0389 U.S.A.Tel (800) 227-HACH(800) 227-4224(U.S.A. o

Contact Information46HACH LANGE S.L.U.Edif. Arteaga CentrumC/Larrauri, 1C- 2ª Pl.E-48160 Derio/VizcayaTel. +34 94 657 33 88Fax +34 94 657 33 97info@ha

5Section 1 SpecificationsSpecifications are subject to change without notice. Table 1 SpecificationsGeneralRange Selectable, 0 to 35, 0 to 70, 0 to 3

Specifications6Radio frequency interferenceThis Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regula

7Section 2 General information2.1 Safety informationPlease read this entire manual before unpacking, setting up or operating this equipment. Pay atten

General information82.2 Theory of operationRespirometric Biochemical Oxygen Demand (BOD) is a test done at 20 °C (68 °F) in a controlled environment.

General information92.2.2 Pressure sensor function The BODTrak II is sealed to prevent external atmospheric pressure changes in the test bottle. Press